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BCHEM 362 — INTEGRATED LABORATORY METHODS II

BSc BSc Biochemistry · Kwame Nkrumah University of Science and Technology

Food analysis practicals: proximate analysis of a food sample to include determination of moisture, ash, protein, crude fibre, non-protein nitrogen, nitrogen-free extract, oils and fats. Analytical biochemistry practicals: determination of the iron content of some selected vegetables by atomic absorption/flame photometry, experiments on chromatography to include adsorption chromatography for separation of plant pigments, experiments on thin layers chromatography for separation of lipids using one solvent and two solvent systems. Demonstration of HPLC and GLC Experiments on rapid isolation of DNA from plant and animal sources. DNA characterisation by agarose gel electrophoresis. Fermentation and biotechnology practicals: Lactic acid fermentation and yoghurt production from cow milk and plant milk sources; cheese production from milk; lactic acid fermentation of some local vegetables; alcohol fermentation – ethanol from sugar/sugar cane; malt from various indigenous cereals – comparison of wort from these cereals; wine production from different carbohydrate sources; solid state fermentation of traditional foods – kenkey, ogi, fish, etc.

Credits
0
Level
Level 300

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Course details from BSc. Biochemistry - Department of Biochemistry & Biotechnology.