BCMB 403 — Molecular Biotechnology & Applications
Biochemistry, Cell and Molecular Biology · University of Ghana
Tools of molecular biology: Agarose and polyacrylamide gel electrophoresis; Northern and Southern blots and hybridization analysis; Western blots and protein detection; PCR and RAPD, RFLP. Purification and characterization of nucleic acids: Principles for extraction and purification; concentration and molecular weight determination; species differentiation (RNA/DNA, single/double stranded nucleic acids). Modifying enzymes: Restriction endonucleases; other nucleases (DNAse, RNAse); ligases; polymerases. Recombinant DNA technology: Cloning and expression vectors, recombinant molecules and transformation systems (prokaryotic and eukaryotic hosts); colony screening, plasmid isolation and characterisation; transduction and conjugation. Nucleotide sequencing and mutagenesis: Sequencing of end labelled DNA by base specific chemical cleavage (Maxam and Gilbert) and analysis of primed enzymatic synthesis (Sanger); deletion and insertion mutagenesis. Gene expression detection: principles of RT-PCR, real time RT-PCR, microarrays. Applications: medicine, agriculture and industry.
- Credits
- 2
- Level
- Level 400
- Semester
- Semester One
Other courses on this programme
- BCMB 400 — Project
- BCMB 401 — Protein Chemistry I
- BCMB 402 — Protein Chemistry II
- BCMB 404 — Immunology and Immunochemistry
- BCMB 405 — Cell Signalling
- BCMB 406 — Molecular Genetics
- BCMB 407 — Cell & Molecular Biology Practical Ii
- BCMB 408 — Entrepreneurship For Innovations In Biosciences
- BCMB 409 — Biochemistry of Parasites
- BCMB 410 — Seminars and Scientific Writing
- BCMB 411 — Clinical Biochemistry
- BCMB 414 — Plant Biochemistry
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Start freeCourse details from University of Ghana Volume 3 Handbook for the Bachelor's Degree: Course Descriptions for Programmes in the Sciences (2017).