BCMB 200 — Practical Biochemistry I
Biochemistry, Cell and Molecular Biology · University of Ghana
Acid-Base Reactions: Titration; pH measurement; buffer preparation; determination of pK. Acid-base reactions; buffers, chromatography, qualitative analysis of carbohydrates, proteins and lipids. Quantitative analysis of proteins: methods for protein estimation (Folin-Lowry, Biuret, Ultraviolet absorption); determination of amino acids (ninhydrin method); preparation, purification and standardization of proteins (serum proteins, cytochrome C). Separation Methods: Paper and gel electrophoresis; chromatography (Paper, TLC, column). Quantitative analysis of carbohydrates: Estimation of glucose (Folin-Wu); isolation of glycogen, determination of rate of hydrolysis and chromatography of hydrolysis products. Quantitative analysis of lipids: Solubility; emulsification; determination of iodine number and acid value; separation of serum lipids.
- Credits
- 3
- Level
- Level 200
- Semester
- Semester Two
Other courses on this programme
- BCMB 201 — Structure and Function Of Biomolecules
- BCMB 202 — Cell Biology I
- BCMB 203 — Principles of Biochemical Techniques
- BCMB 204 — Enzymology
- BCMB 206 — Spectroscopic And Radioisotopic Techniques
- BCMB 207 — Veterinary Biochemistry I
- BCMB 208 — Veterinary Biochemistry II
- BCMB 212 — Veterinary Biochemistry III
- BCMB 301 — Intermediary Metabolism
- BCMB 302 — Cell Biology II
- BCMB 303 — Molecular Biology I
- BCMB 304 — Molecular Biology II
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Start freeCourse details from University of Ghana Volume 3 Handbook for the Bachelor's Degree: Course Descriptions for Programmes in the Sciences (2017).