BCMB 203 — Principles of Biochemical Techniques
Biochemistry, Cell and Molecular Biology · University of Ghana
Chromatography: Partition coefficient and chromatographic systems. Basis of separation: adsorption and partition (polarity); ion-exchange (ionic nature), exclusion/gel (molecular size and shape). Principles and applications (HPLC, FPLC, GLC, TLC, Paper, Chromatofocusing and two-dimensional electrophoresis). Analytical aspects: retention time and volume, capacity ratio, peak resolution theoretical plates/plate height, peak capacity, internal and external standardization and analyte quantitation. Centrifugation: Basic principles of sedimentation, RCF value, relationship between v, s and G. Centrifuges and rotors (types and uses). Preparative centrifugation: differential and density gradient; preparation of gradients, recovery and monitoring of fractionates. Analytical centrifugation: determination of relative molar mass (sedimentation velocity and equilibrium methods), purity and shape of macromolecules. Electrophoresis: General principles. Low voltage thin sheets (paper, cellulose acetate, thin layer) and high voltage gels (agarose, polyacrylamide - native, gradient and SDS-PAGE). Applications; purity and molecular weight determination of proteins and nucleic acids, DNA sequencing. Iso-electric focusing and isotachophoresis.
- Credits
- 2
- Level
- Level 200
- Semester
- Semester One
Other courses on this programme
- BCMB 200 — Practical Biochemistry I
- BCMB 201 — Structure and Function Of Biomolecules
- BCMB 202 — Cell Biology I
- BCMB 204 — Enzymology
- BCMB 206 — Spectroscopic And Radioisotopic Techniques
- BCMB 207 — Veterinary Biochemistry I
- BCMB 208 — Veterinary Biochemistry II
- BCMB 212 — Veterinary Biochemistry III
- BCMB 301 — Intermediary Metabolism
- BCMB 302 — Cell Biology II
- BCMB 303 — Molecular Biology I
- BCMB 304 — Molecular Biology II
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Start freeCourse details from University of Ghana Volume 3 Handbook for the Bachelor's Degree: Course Descriptions for Programmes in the Sciences (2017).