BCMB 204 — Enzymology
Biochemistry, Cell and Molecular Biology · University of Ghana
Introduction to Enzymes: Comparison of chemical and enzyme catalysis, Activation energy and transition state, Free energy change, Chemical equilibria, Active site, Substrate specificity, Enzyme classification, enzyme assays, linked or coupled. Factors affecting Enzyme Activity: Reaction rate (v), Effect of [S], [E], T, pH on enzyme activity; coenzyme, prosthetic groups. Enzyme Kinetics and Inhibition: Michaelis - Menten model, Graphical representation of data (e.g. Lineweaver - Burk and Hanes plots) Enzyme inhibition: Reversible (Competitive, noncompetitive, uncompetitive) and irreversible Control of Enzyme Activity: Feedback regulation, allosteric enzymes, isozymes, covalent modification, activation, regulation of synthesis and breakdown (eg. lac operon, tryptophan biosynthesis). Enzyme Purification: Cell disruption techniques, general purification strategy, enzyme assays, units of enzyme activity. Application of enzymes in health, agriculture and industry
- Credits
- 2
- Level
- Level 200
- Semester
- Semester Two
Other courses on this programme
- BCMB 200 — Practical Biochemistry I
- BCMB 201 — Structure and Function Of Biomolecules
- BCMB 202 — Cell Biology I
- BCMB 203 — Principles of Biochemical Techniques
- BCMB 206 — Spectroscopic And Radioisotopic Techniques
- BCMB 207 — Veterinary Biochemistry I
- BCMB 208 — Veterinary Biochemistry II
- BCMB 212 — Veterinary Biochemistry III
- BCMB 301 — Intermediary Metabolism
- BCMB 302 — Cell Biology II
- BCMB 303 — Molecular Biology I
- BCMB 304 — Molecular Biology II
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Start freeCourse details from University of Ghana Volume 3 Handbook for the Bachelor's Degree: Course Descriptions for Programmes in the Sciences (2017).