Questora

BCMB 312 — Cell & Molecular Biology Practical I

Biochemistry, Cell and Molecular Biology · University of Ghana

Preparation of microbial cells: Safety precautions; sterility; types of growth media (liquid, solid); identification and classification of microorganisms: morphology, Gram stain, biochemical tests; measurement of microbial concentrations; comparison of growth rate in differently constituted media; selective action of antibiotics. Cell fractionation: Cytoplasm, Nuclear, Mitochondria, Mitoplast; Assay for enzyme markers e.g. succinate dehydrogenase. Use of protein assay to monitor cell growth: Cell growth; cell density; centrifugation; protein determination. Cell Behaviour: Cell interactions; kinetics of cell pairing; phagocytosis; kinetics of phagocytosis; exocytosis. DNA technology: Isolation, purification and manipulation of DNA; RFLP, PCR, Hybridization.

Credits
3
Level
Level 300
Semester
Semester Two

Other courses on this programme

Practising BCMB 312 with Questora

Upload your own slides, notes and past papers for BCMB 312 and Questora builds mock exams, practice questions and a revision plan from them.

Start free

Course details from University of Ghana Volume 3 Handbook for the Bachelor's Degree: Course Descriptions for Programmes in the Sciences (2017).